Scientists Discover the Origins of Genomic 'Dark Matter'
Wednesday, 18 September 2013
A duo of scientists at Penn State University has achieved a major milestone in understanding how genomic "dark matter" originates. This "dark matter" – called non-coding RNA – does not contain the blueprint for making proteins and yet it comprises more than 95 percent of the human genome. The researchers have discovered that essentially all coding and non-coding RNA originates at the same types of locations along the human genome. The team's findings eventually may help to pinpoint exactly where complex-disease traits reside, since the genetic origins of many diseases reside outside of the coding region of the genome.
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A duo of scientists at Penn State
University has
achieved a major milestone in
understanding
genomic "dark matter" --
called non-coding RNA.
This "dark matter" is
difficult to detect and no
one knows exactly what it is doing or
why it is
there in our genome, but scientists
suspect it may
be the source of inherited diseases.
This research
achievement may help to pinpoint
exactly where
complex-disease traits reside in the
human
genome. This illustration shows, in the
upper left
corner, a chromosome – a densely
compressed
package containing one long, continuous
strand
of DNA. The DNA is pervasively
transcribed into
RNA, but only a very small fraction of
the RNA
has the instructions (or codes) for
making proteins.
The green circles in this illustration
represent places
along the strand of DNA where
transcription
originates. New research led by B.
Franklin Pugh
of Penn State University shows that
essentially all
RNA, whether or not it codes for
proteins, originates
at the same types of locations along
the strand
of DNA. The findings eventually may
help to
pinpoint exactly where complex-disease
traits
reside, since the genetic origins of
many diseases
reside outside of the coding region of
the genome.
Credit: National
Institutes of Health and B.
Franklin Pugh, Penn State University.
|
In their research, Pugh and Venters set out to identify the precise location of the beginnings of transcription – the first step in the expression of genes into proteins.
"During transcription, DNA is copied into RNA – the single-stranded genetic material that is thought to have preceded the appearance of DNA on Earth – by an enzyme called RNA polymerase and, after several more steps, genes are encoded and proteins eventually are produced," Pugh explained.
He added that, in their quest to learn just where transcription begins, other scientists had looked directly at RNA. However, Pugh and Venters instead determined where along human chromosomes the proteins that initiate transcription of the non-coding RNA were located.
"We took this approach because so many RNAs are rapidly destroyed soon after they are made, and this makes them hard to detect," Pugh said.
"So rather than look for the RNA product of transcription we looked for the 'initiation machine' that makes the RNA. This machine assembles RNA polymerase, which goes on to make RNA, which goes on to make a protein."
Pugh added that he and Venters were stunned to find 160,000 of these "initiation machines," because humans only have about 30,000 genes.
"This finding is even more remarkable, given that fewer than 10,000 of these machines actually were found right at the site of genes. Since most genes are turned off in cells, it is understandable why they are typically devoid of the initiation machinery."
The remaining 150,000 initiation machines – those Pugh and Venters did not find right at genes – remained somewhat mysterious.
"These initiation machines that were not associated with genes were clearly active since they were making RNA and aligned with fragments of RNA discovered by other scientists," Pugh said.
"In the early days, these fragments of RNA were generally dismissed as irrelevant since they did not code for proteins."
Pugh added that it was easy to dismiss these fragments because they lacked a feature called polyadenylation – a long string of genetic material, adenosine bases – that protect the RNA from being destroyed. Pugh and Venters further validated their surprising findings by determining that these non-coding initiation machines recognized the same DNA sequences as the ones at coding genes, indicating that they have a specific origin and that their production is regulated, just like it is at coding genes.
"These non-coding RNAs have been called the 'dark matter' of the genome because, just like the dark matter of the universe, they are massive in terms of coverage – making up over 95 percent of the human genome. However, they are difficult to detect and no one knows exactly what they all are doing or why they are there," Pugh said.
"Now at least we know that they are real, and not just 'noise' or 'junk.' Of course, the next step is to answer the question, 'what, in fact, do they do?'"
Pugh added that the implications of this research could represent one step towards solving the problem of "missing heritability" – a concept that describes how most traits, including many diseases, cannot be accounted for by individual genes and seem to have their origins in regions of the genome that do not code for proteins.
"It is difficult to pin down the source of a disease when the mutation maps to a region of the genome with no known function," Pugh said.
"However, if such regions produce RNA then we are one step closer to understanding that disease."
Source: Penn State
Contact: Barbara K. Kennedy
Reference:
Genomic organization of human
transcription initiation complexes
Bryan J. Venters & B.
Franklin Pugh
Nature (2013), doi:10.1038/nature12535.........
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