Neuronal-like Cell Differentiation of Non-adherent BMSCs

Neuronal-like Cell Differentiation of Non-adherent BMSCs

Thursday, 05 September 2013

Cells co-labeled with both β-galactosidase and

NeuN were seen in ischemia brain following

transplantation of non-adherent bone marrow

cell-derived mesenchymal stem cells from

β-galactosidase transgenic mice (immunohisto-

chemical staining, ×1000). Credit: Neural

Regeneration Research.

It is widely believed that bone marrow mesenchymal stem cells are highly adherent fibroblastic cells, defined as colony-forming unit-fibroblasts. Nevertheless, a few reports have shown that the non-adherent bone marrow cells can give rise to colony-forming unit-fibroblasts in vitro, and possess a certain differentiation potential.

According to a recent study from Dr. Xiaoming Ben and colleagues, non-adherent bone marrow cell-derived mesenchymal stem cells from C57BL/6J mice cultured using the "pour-off" method developed colony-forming unit-fibroblasts, and could be expanded by supplementation with epidermal growth factor. The non-adherent bone marrow cell-derived mesenchymal stem cells exposed to basic fibroblast growth factor/epidermal growth factor/nerve growth factor expressed the neuron specific markers, neurofilament-200 and NeuN, in vitro.

Non-adherent bone marrow cell-derived mesenchymal stem cells from β-galactosidase transgenic mice were also transplanted into focal ischemic brain (right corpus striatum) of C57BL/6J mice. Cells co-labeled with both β-galactosidase and NeuN were seen by double immunohistochemical staining. These findings, published in the Neural Regeneration Research, suggest that the non-adherent bone marrow cell-derived mesenchymal stem cells could differentiate into neuronal-like cells in vitro and in vivo, which can be used as seed cells for the treatment of nervous system diseases.

Source: Neural Regeneration Research 

Contact: Meng Zhao

Reference:

Neuronal-like cell differentiation of non-adherent bone marrow cell-derived mesenchymal stem cells

Yuxin Wu, Jinghan Zhang, Xiaoming Ben

Neural Regen Res. 2013, 8(22): 2078-2085

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ZenMaster

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