Paired Genes in Stem Cells Shed New Light on Gene Organization and Regulation
Tuesday, 05 February 2013
Whitehead Institute researchers have determined that DNA transcription, the process that produces messenger RNA (mRNA) templates used in protein production, also runs in the opposite direction along the DNA to create corresponding long noncoding RNAs (lncRNAs). Moreover, the mRNAs and lncRNAs are transcribed co-ordinately as stem cells differentiate into other cell types. This surprising finding could redefine our understanding of gene organization and its regulation.
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In the transcription of protein-coding
messenger RNA
(mRNA) and long noncoding RNA (lncRNA)
genes
(shown as wide tan lines), the
transcription machinery
(shown as green ovals) initiates
transcription at an active
promoter and commonly runs opposite
directions. This
indicates that the long-established
protein-coding genes
actually exist in the genome as gene
pairs with lncRNA
genes and leads to coordinated
production of both mRNAs
(shown in blue) and lncRNAs (shown in
red). Credit: Image
by
Alla Sigova.
|
"At any one of the 20,000 protein-coding genes that are active in human stem cells, a lncRNA gene located upstream is also transcribed. So much effort has gone into studying protein-coding genes, and yet we have missed this concept that all protein-coding genes come in mRNA/lncRNA pairs. If you activate the mRNA gene, you're going to activate the lncRNA gene."
Young and his lab report their findings this week in the Proceedings of the National Academy of Sciences (PNAS).
Until now, scientists thought transcription machinery attaches to DNA at certain points called promoters and moves just in one direction along the DNA to create mRNAs from protein-coding genes. Other RNAs that are not protein templates, including lncRNAs, are also created by transcription, but despite their important roles in in regulation of gene expression, development and disease, scientists knew little about how lncRNA transcription is initiated or where most lncRNA genes reside in the genome.
By looking at human and mouse embryonic stem cells, researchers in the Young lab found something astonishing — most lncRNA genes are located adjacent mRNA genes, and the transcription of about 65% of lncRNAs originates at active promoters associated with these mRNAs' genes and runs "upstream" and in the opposite direction from the promoter.
When the transcription machinery attaches to a promoter, it seems that it is just as likely to move in one direction and transcribe the mRNA as it is to move in opposite direction and transcribe the neighbouring lncRNA.
The researchers also noticed that as embryonic stem cells begin differentiating into other cell types, the mRNA/lncRNA pairs are regulated in the same way — the transcription of paired mRNAs and lncRNAs is up regulated or down regulated together. This further confirms the relationship between the transcription of mRNAs and lncRNAs.
"I think it's quite a breakthrough," says Alla Sigova, who is a postdoctoral researcher in the Young lab and a co-author of the paper in PNAS.
"This provides a unifying principle for production of mRNAs and lncRNAs, and may lead to new insights into lncRNA mis-regulation in disease. For example, some mRNAs are up- or down regulated in cancer, so their lncRNA partners could potentially contribute to cancer."
Contact: Nicole Rura
Reference:
Divergent transcription of long noncoding RNA/mRNA gene pairs in embryonic stem cells"
Alla A. Sigova, Alan C. Mullen, Benoit Molinie, Sumeet Gupta, David A. Orlando, Matthew G. Guenther, Albert E. Almada, Charles Lin, Phillip A. Sharp, Cosmas C. Giallourakis, and Richard A. Young
PNAS, February 4, 2013, doi:10.1073/pnas.1221904110
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