Researchers Grow Cardiac Tissue on 'Spider Silk' Substrate
Saturday, 11 April 2015
Genetically engineered fibres of the protein spidroin, which is the construction material for spider webs, has proven to be a perfect substrate for cultivating heart tissue cells, Moscow Institute of Physics and Technology researchers found. They discuss their findings in an article that has recently come out in the journal PLOS ONE.
These are heart tissue cells grown on a matrix,
stained with fluorescent markers. Credit: ©
Alexander Teplenin et al./PLOS ONE.
This is a spidroin fibre matrix captured with a
microscope. Credit: Alexander Teplenin et al./
For this purpose, they seeded isolated neonatal rat cardiomyocytes on fibre matrices. During the experiment, the researchers monitored the growth of the cells and tested their contractibility and the ability to conduct electric impulses, which are the main features of normal cardiac tissue.
The monitoring, carried out with the help of a microscope and fluorescent markers, showed that within three to five days a layer of cells formed on the substrate that were able to contract synchronously and conduct electrical impulses just like the tissue of a living heart would.
"We can answer positively all questions we put at the beginning of this research project," Professor Agladze says.
"Cardiac tissue cells successfully adhere to the substrate of recombinant spidroin; they grow forming layers and are fully functional, which means they can contract co-ordinately."
Contact: Stanislav Goryachev
Functional Analysis of the Engineered Cardiac Tissue Grown on Recombinant Spidroin Fiber Meshes
PLOS ONE, March 23, 2015, DOI:10.1371/journal.pone.0121155
For more on stem cells and cloning, go to CellNEWS athttp://cellnews-blog.blogspot.com/