Tuesday, 7 October 2014

Vesicles Influence the Function of Nerve Cells

Neurons react to the transmission activity of exosomes on three fundamental levels
Tuesday, 07 October 2014

The JGU researchers were able to show that
exosomes are absorbed by the nerve cells and
thus help protect these against stress. Credit:
Institute of Molecular Cell Biology. 
Tiny vesicles containing protective substances which they transmit to nerve cells apparently play an important role in the functioning of neurons. As cell biologists at Johannes Gutenberg University Mainz (JGU) have discovered, nerve cells can enlist the aid of mini-vesicles of neighbouring glial cells to defend themselves against stress and other potentially detrimental factors. These vesicles, called exosomes, appear to stimulate the neurons on various levels: they influence electrical stimulus conduction, biochemical signal transfer, and gene regulation. Exosomes are thus multifunctional signal emitters that can have a significant effect in the brain.

Neurons (blue) which have absorbed exosomes
(green) have increased levels of the enzyme
catalase (red), which helps protect them against
peroxides. Credit: Institute of Molecular Cell
Biology, JGU.
The researchers in Mainz already observed in a previous study that oligodendrocytes release exosomes on exposure to neuronal stimuli. These exosomes are absorbed by the neurons and improve neuronal stress tolerance. Oligodendrocytes are a type of glial cell and they form an insulating myelin sheath around the axons of neurons. The exosomes transport protective proteins such as heat shock proteins, glycolytic enzymes, and enzymes that reduce oxidative stress from one cell type to another, but also transmit genetic information in the form of ribonucleic acids.

"As we have now discovered in cell cultures, exosomes seem to have a whole range of functions," explained Dr. Eva-Maria Krämer-Albers. By means of their transmission activity, the small bubbles that are the vesicles not only promote electrical activity in the nerve cells, but also influence them on the biochemical and gene regulatory level.
Cultivated neurons on a multielectrode array
chip: the electrodes register the electrical
impulses of the neurons. Credit: Institute of
Physiology, Mainz University Medical Center.

"The extent of activities of the exosomes is impressive," added Krämer-Albers. The researchers hope that the understanding of these processes will contribute to the development of new strategies for the treatment of neuronal diseases. Their next aim is to uncover how vesicles actually function in the brains of living organisms.

Contact: Dr. Eva-Maria Krämer-Albers

Multifaceted effects of oligodendroglial exosomes on neurons: impact on neuronal firing rate, signal transduction and gene regulation
Dominik Fröhlich, Wen Ping Kuo, Carsten Frühbeis, Jyh-Jang Sun, Christoph M. Zehendner, Heiko J. Luhmann, Sheena Pinto, Joern Toedling, Jacqueline Trotter and Eva-Maria Krämer-Albers
Philosophical Transactions of the Royal Society B, 18 August 2014 DOI: 10.1098/rstb.2013.0510

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